Journal: Journal of Neurotrauma

Article Title: Mild Traumatic Brain Injury Decreases Spatial Information Content and Reduces Place Field Stability of Hippocampal CA1 Neurons

doi: 10.1089/neu.2019.6766

Figure Lengend Snippet: Isolation and classification of neurons based on waveform and firing rate characteristics. (A) Principal components (PC1 and PC2) plot showing three isolated cluster clouds of waveforms of neuronal activity. (B) Average waveforms of the neural activity for each cluster are shown. Four parameters of the waveform are indicated, the minimum voltage (min), the maximum voltage (max), and two measures of width: the peak latency and full width at half maximum (FWHM). Example waveforms from two putative pyramidal neurons (green and blue) are shown. The multi-unit activity in pink was excluded from further analysis. (C) Scatterplot of the 248 neurons from sham animals (n = 4) plotted by the peak latency on the ordinate and the firing rate on the abscissa. The neurons with a peak latency of 0.4 to 0.8 msec and a firing rate of <5 Hz are considered pyramidal neurons. The population of putative pyramidal neurons is indicated by the blue box, whereas inhibitory neurons are enclosed within the red box. Pie chart illustrating the distribution of identified cells types taken from within the two inset boxes is shown. Place cells were identified as indicated by having low firing rates, wide action potentials, and spatial information in bits/spike of ≥0.99. (D) Scatterplot and pie chart of the 240 neurons identified in mild lateral fluid percussion injured (mFPI) animals.

Article Snippet: Place cells were identified as indicated by having low firing rates, wide action potentials, and spatial information in bits/spike of ≥0.99. (D) Scatterplot and pie chart of the 240 neurons identified in mild lateral fluid percussion injured (mFPI) animals.

Techniques: Isolation, Activity Assay